Development of Biocatalytic Processes
Development of Production Processes
BC2 Technology: Enzymatic
Splitting of CPC
Biotech Concept's Team & BC2 Technology
Biotech
Concepts Ltd. (BC) has experience in the enzymatic conversion of
cephalosporin C (CPC) into 7-aminocephalosporanic acid (7-ACA) which is
a key intermediate for the production of antibiotics.
BC has developed novel high yielding recombinant strains for the
production of D-amino acid oxidase (DAO) and the glutaryl-7-ACA acylase
(GAC), used in the BC2 process.
BC's know-how covers all important aspects of the industrial production
of 7-ACA,in particular the production of the immobilized BC2 enzymes.
The New System: BC's BC2 Strains
BC has succeeded in eliminating the main draw backs of traditional BC2 enzyme production by using one very productive host for both enzymes resulting in a highly efficinet enzyme production technology.
- Robust and reliable expression with low failure
rate, no induction procedure
- Low cost fermentation medium (no complex-media)
- Straightforward fed-batch fermentations
- Superior genetic stability
- No esterase side activity
- Same downstream processing equipment for both enzymes
- No harvest decanter and chromatography in DSP
- Enzyme for 1t of 7-ACA needs just 20-33 L DAO and 8 -20 L GAC fermentation broth (traditional systems needs up to 800 L and up to 220L fermentation broth, respectively)
Small size of the fermentation volume (ca. 3 m³ per batch) leads
to
- low investment in plant (savings approx. 2 million USD*)
- low investment in utilities
- low labor cost
- easy start up, maintainance and handling
- easy outsourcing of enzyme manufacturing
Cost-savings (process and plant maintainance)approx. 600,000 USD/y*
*Savings based on a production of 300-500t 7-ACA/a
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Enzymatic Splitting of 7-ACA to DACA
Biotech Concepts (BC) has the know-how and
resources to support the implementation of the enzymatic splitting of 7-ACA to desacetyl-7-ACA (DACA), covering the process from the enzyme production to the finished DACA.
CPC-3-actetyl esterase (CAE) expressing strains can be generated by means of genetic engineering and cloning.
We can set up the required lab data and experimental work on the process in laboratories of our Swiss and pan-European network and/or we can advise and supervise the setting up of this work in the facilities of the partner.
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Enzymatic Splitting of PenG to 6-APA
Biotech Concepts (BC) has know-how and resources
to support the implementation of the enzymatic splitting of PenG to
6-amino penicillic acid (6-APA), covering the process from the enzyme production to the finished product.
We can provide an industrially used proprietary PenG acylase (PGA) expressing bacterial strain.
We can set up the required lab data and experimental work on the process in laboratories of our Swiss and pan-European network and/or we can advise and supervise the setting up of this work in the facilities of the partner.
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